Composite
Part:BBa_K3941005:Design
Designed by: Can Bora Ciner Group: iGEM21_Saint_Joseph (2021-10-17)
pET29b+EGII
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 22
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 93
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 22
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 22
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is created based on only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization has carried out to increase production in Escherichia coli bacterium. His-tag was added to 3' end of the sequence to help purification of protein product. A single aminoacid mutation has been studied as Aspartic acid aminoacid in 185 aminoacid position is changed with Serine aminoacid.
Source
The source of this part is Trichoderma reesei endoglucanase II gene.